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KMID : 0613820090190091314
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Journal of Life Science
2009 Volume.19 No. 9 p.1314 ~ p.1320
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Evaluation of Cell Death and the Reduction of ERK Phosphorylation in Non-Small Cell Lung Cancer Cells after Exposure to Sodium Butyrate
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Park Ji-Eun
Lee Seung-Gee
Lim Hyun-Ju
Kim Ji-Young
Chung Jin-Yong
Kim Yoon-Jae
Lee Chang-Hun
Lee Min-Ki
Yoo Ki-Soo
Yuh Young-Hyun
Kim Jong-Min
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Abstract
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Histone deacetylase inhibitor (HDACI) is a new promising candidate as an antineoplastic agent for the treatment of solid and hematologic malignancies. In order to evaluate cell death and to elucidate the related mechanism(s) in NSCLC cells after HDACI, sodium butyrate (SB), a representative HDACI, was used to treat H460 cells for 48 hrs. SB exposure resulted in a significant reduction of cell viability at concentrations below 7.5 mM, and about 50% of cell death occurred at 20 mM. The types of cell death induced by SB were both apoptosis and necrosis, evaluated by Annexin-V staining combined with propidium iodide. SB treatment significantly evoked G2/M cell cycle arrest and subsequently induced cell death with caspase-dependent manner. While ERK protein content was not altered after SB, phosphorylated forms of ERK were markedly reduced. Taken together, SB is significantly able to induce cell death in NSCLC cell line H460, and it is suggested that the reduction of ERK phosphorylation might be closely involved in the cancer cell death mechanism initiated by HDACI.
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KEYWORD
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Sodium butyrate, apoptosis, caspase, p53, ERK, lung cancer cell
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